Uso del músculo temporal en reconstrucción periorbitaria post exéresis de carcinoma epidermoide cutáneo del globo ocular izquierdo / Use of the temporal muscle in post exeresis periorbital reconstruction of cutaneous epidermoid carcinoma of the left eyeball

Introducción. El carcinoma epidermoide cutáneo (CEC) es la segunda neoplasia cutánea más frecuente después del carcinoma basocelular. La incidencia del CEC ha aumentado de forma considerable durante los últimos 20 años y predicen un incremento en la próxima década. La mayoría de los CEC están localizados y se resuelven habitualmente mediante la extirpación quirúrgica u otros procedimientos locales. El uso del músculo temporal es una alternativa quirúrgica para corregir el defecto periorbitario tras la extirpación del CEC. Objetivo. Evaluar el resultado de la cobertura del músculo temporal en la corrección del defecto periorbitario. Material y métodos. Se presenta un caso quirúrgico de un paciente masculino, 62 años, que presenta una gran lesión tumoral que compromete el globo ocular, región orbitaria y periorbitaria izquierda, acompañado de dolor, anemia, astenia y pérdida ponderal de aproximadamente 20 libras. Con una evolución de 6 años. Conclusión. El uso del músculo temporal es una alternativa eficaz en la reconstrucción de lesiones craneofaciales, que ha sido utilizado por más de 100 años.

Introduction. Skin epidermoid carcinoma (SEC) is the second most common skin neoplasm after basal cell carcinoma. The incidence of SEC has increased considerably over the past 20 years and predicts an increase over the next decade. Most SECs are located and usually resolved by surgical removal or other local procedures. The use of the temporal muscle is a surgical alternative to correct the peri-orbital defect after removal of the SEC. Objective. To evaluate the result of temporal muscle coverage in the correction of the peri-orbital defect. Material and methods. There is a surgical case of a male patient, 62 years old who has a large tumor injury that compromises the eyeball, orbital region and left periorbital. Accompanied by pain, anemia, asthenia, and weight loss approximately 20 pounds. With an evolution of 6 years. Conclusion. The use of the temporalis muscle is an effective alternative in the reconstruction of craniofacial lesions that has been used for more than 100 years

Antimicrobial activity of Musca domestica cecropin -4 ( mdCec 4 ) against Acinetobacter baumannii / 中华微生物学和免疫学杂志

Objective To study the antimicrobial activity of chemically synthesized Musca domes-tica cecropin-4 (mdCec4) against Acinetobacter baumannii. Methods Minimum inhibitory concentration (MIC) and minimum bactericidal concentration(MBC) of mdCec4 against Acinetobacter baumannii were de-tected by microdilution method. Dynamic time-kill curve was monitored by microplate reader. Effects of dif-ferent influencing factors,such as NaCl,trypsin and pH value,on the antimicrobial activity of mdCec4 were assessed. The ultrastructure of Acinetobacter baumannii exposed to the MIC of mdCec4 was observed under scanning electron microscope (SEM). Results The MIC and MBC of mdCec4 against Acinetobacter bau-mannii were 4 μg/ml and 8 μg/ml,respectively. The dynamic time-kill curve revealed that mdCec4 had a high antimicrobial activity within 24 hours. The antimicrobial activity of mdCec4 was not affected by NaCl concentration ranged from 0 to 200 mmol/L, but could be inhibited by trypsin at a concentration of 1.0 mg/ml. Among Tris-HCl buffers with different pH values (6.5, 7.5, 8.8), the optimum condition for mdCec4 was pH8.8. Results of SEM showed that the degree of bacterial cell injury was positively correlated with exposing time. Conclusion This study shows that the newly discovered mdCec4 has a better antimicro-bial activity against Acinetobacter baumannii as it can eliminate Acinetobacter baumannii within a short period of time and its antimicrobial ability can last more than 24 hours. Moreover,mdCec4 has the characteristic of good stability in conditions of different concentrations of NaCl and Trypsin as well as various pH values. SEM observation shows that the MIC of mdCec4 can destroy the integrity of bacterial membrane resulting in a re-lease of a large number of cell contents, which finally lead to the death of Acinetobacter baumannii. This study provides references for further study of the antimicrobial mechanism of mdCec4 against Acinetobacter baumannii.

Uso do melhor preditor linear não-viesado (BLUP) na predição de híbridos em feijão / Using the best linear unbiased predictor (BLUP) for predicting hybrid beans

O objetivo deste trabalho foi avaliar a eficiência do BLUP (Melhor preditor linear não-viesado) para predizer o desempenho de híbridos com base nos genitores, comparando os resultados obtidos com as análises dialélicas. Para tanto, foram realizados cruzamentos dialélicos entre seis genótipos de feijão: Xan 159, Pérola, BAF 63, IPR Uirapuru, BRS Supremo e BRS Valente. Todas as plantas foram avaliadas quanto aos caracteres de importância agronômica. Os dados obtidos foram submetidos à análise dialélica modelo 1 método I de Griffing (1956) que estima a capacidade geral e específica de combinação, e utilizando o BLUP foram obtidos os valores preditos dos genitores e de seus híbridos. Após foram realizadas análises de correlação entre os BLUP's e os quadrados médios da capacidade geral e específica de combinação. Contudo, concluímos que associação do BLUP às análises dialélicas possibilita identificar populações segregantes promissoras, permitindo rapidez no desenvolvimento de novas cultivares.

The aim of this study was to evaluate the efficiency of BLUP (best linear unbiased predictor) to predict hybrid performance based on parents, comparing the results obtained with the Diallel analysis. For this, were performed diallel crosses among six common bean genotypes: Xan 159, Pérola, BAF 63, IPR Uirapuru, Supreme BRS and BRS Valente. All plants were evaluated for important agronomic characteristics. The data were subjected to diallel analysis model I and method I of Griffing (1956) that estimated the general ability and specific combining, and using BLUP were obtained the values predicted of parents and their hybrids. After correlation analysis was performed between the BLUP's and the mean squares of general ability and specific combining. However, we conclude that the association BLUP Diallel analysis enables the identification of segregating populations, enabling rapid development of new cultivars.

Propriedades químicas de um Argissolo tratado sucessivamente com composto de lixo urbano / Chemical properties of an Ultisol successively treated with municipal waste compost

Com o objetivo de avaliar as alterações em algumas propriedades químicas do solo sob adição sucessiva de composto de lixo urbano, conduziu-se um experimento a campo, em um solo Argissolo Vermelho de textura média na Estação Experimental Agronômica da UFRGS. Os tratamentos constaram de cinco doses de composto (0, 20, 40, 80 e 160t ha-1), de um tratamento com adubo mineral e uma testemunha (Calagem e NPK), com aplicações sucessivas por um período de quatro anos. A adição de composto no solo aumentou o pH, CTC, C orgânico, N total, P e Na extraíveis, bem como da relação de adsorção de sódio (RAS), Ca e Mg trocáveis e o teor de K extraível após a terceira aplicação. O Al trocável teve os seus teores diminuídos após aplicações sucessivas de composto. Os resultados permitiram concluir que aplicação de até 80t ha-1 por ano de composto de lixo urbano pode ser considerada como adequada para melhorar e, ou, manter as propriedades químicas do solo.

In order to assess the changes in some chemical properties of soil under the successive addition of urban waste compost, it was conducted a field experiment in an Ultisol soil at Agronomic Experimental Station of UFRGS. The treatments consisted of five successive applications of different doses of municipal solid waste compost (0, 20, 40, 80 and 160t ha-1) for a period of four years and a comparative treatment with mineral fertilizer and a control (without compost and fertilization). The addition of the compost increased soil pH, CEC, organic C, total N, extractable P and Na and the sodium adsorption ratio (SAR), calcium, and magnesium and extractable K after the third application. The exchangeable Al had their levels reduced by successive applications of compost. The results showed that application of up to 80t ha-1 yr-1 of urban waste compost can be the dose recommended to improve and maintain the chemical properties of soil.

Citologia esfoliativa convencional versus citologia em meio líquido para prevenção e diagnóstico precoce do carcinoma escamo celular (CEC) oral / Conventional cytology versus liquid-based cytology for prevention and early diagnosis of Oral Squamous Cell Carcinoma (OSCC)

Objetivando avaliar a concordância, sensibilidade e especificidade diagnóstica dos métodos citológicos:convencional e em meio líquido, foi realizado um estudo de caso-controle, no Ambulatório deCabeça e Pescoço do Hospital de Câncer de Pernambuco, sendo selecionados 182 indivíduos doentese 179 sãos, no período de setembro de 2002 a janeiro de 2004, os quais, permitiram através de assinatura de termo de consentimento livre e esclarecido a realização de exames (clínico/ citológicoesfoliativo - cytobrush) na área carcinomatosa e em área sã, constituindo, respectivamente, o grupo de casos e controles. Os dados obtidos foram analisados através dos softwares estatísticos SPSS(Statistical Package for Social Science) e Epi-Info versão 6.4. Os resultados evidenciaram que os métodosdiagnósticos citológicos apresentaram entre os casos: sensibilidade= 96,9% (IC= 95%: 92,5% a98,8%); especificidade= 75,0% (IC= 95%: 21,9% a 98,7%) e acurácia= 96,3% (IC=95%: 92,5% a 98,5%);e entre os controles: sensibilidade= 91,0% (IC=95%: 84,1% a 95,2%); especificidade = 57,5% (IC=95%:41,0% a 72,6%) e acurácia= 82,7% (IC=95%: 76,3% a 88,0%). A citologia convencional e em base--líquida apresentaram concordância com o diagnóstico histopatológico em mais de 90%. Conclui-se, portanto, que, se bem indicado e executado, o exame citológico pode ser utilizado rotineiramente como exame complementar, pois ambos apresentaram alta sensibilidade e razoável especificidade,cabendo à citologia convencional maior especificidade para o diagnóstico das lesões orais.

The purpose of this study was to evaluate the sensitivity, specificity, and concordance between conventionalcytology and liquid-based cytology. A case-control study was developed at the Head andNeck ambulatory service of Hospital do Cancer de Pernambuco. A hundred and eighty-two patientswith primary Oral Squamous Cellular Carcinoma (OSCC) (case group) and 179 individuals with normalbuccal mucosa (control group) were selected, from September 2002 to January 2004. They agreed tobe submitted to clinical examination and exfoliative cytology of the oral cavity, by signing a documentof consent. The data were analysed by Statistical Package for Social Science (SPSS) and Epi-Info 6.4 program.Among the cases, the cytologic methods demontrated a sensitivity= 96.9% (IC= 95%: 92.5% to98.8%), specificity= 75.0% (IC=95%: 21.9% to 98.7%), and accuracy= 96.3% (IC=95%: 92.5% to 98.5%).Among the controls the results were: sensitivity= 91.0% (IC=95%: 84.1% to 95.2%);, specificity= 57.5%(IC=95%: 41.0% to 72.6%), and accuracy= 82.7% (IC=95%: 76.3% to 88.0%). Conventional cytologyand liquid-based cytology demonstrated a diagnostic concordance with histopathology of more than90%. In conclusion if properly indicated and executed, they can be routinely used as complementarydiagnostic methods. When compared to each other, conventional cytology and liquid-based cytologyshowed a high sensitivity and reasonable specificity, conventional cytology having higher specificity.

Hiperlactatemia durante la cirugía cardiaca pediátrica con circulación extracorpórea / The hyperlactacidemia related to pediatric cardiac surgery using extracorporeal circulation

Introducción: El ácido láctico es considerado como un indicador temprano de supervivencia y/o mortalidad en pacientes que necesitan procedimientos quirúrgicos cardiovasculares. Objetivo: Determinar el comportamiento del ácido láctico en sangre arterial durante la cirugía cardiaca pediátrica y el pronóstico de dichos pacientes al egresar de la unidad de cuidados intensivos. Material y Método: Se realizó un estudio prospectivo, analítico y observacional de 55 pacientes pediátricos que recibieron circulación extracorpórea en el Cardiocentro Pediátrico "William Soler". Se tomaron muestras de sangre arterial una vez realizada la inducción anestésica (ácido láctico inicial), precirculación extracorpórea y al final de ella. Se consideró hiperlactatemia cuando el valor de acido láctico en sangre arterial estuvo por encima de 3 mmol/L durante el intraoperatorio y postoperatorio inmediato. Resultados: La cuantificación del ácido láctico en diferentes momentos del proceder quirúrgico fue significativa para el valor al inicio del intraoperatorio y el valor en circulación extracorpórea, no lo fue así para la relación de éste último con el valor al final de la misma. Se demostró que el tipo de cardiopatía no estuvo asociada con el estado al egreso. Conclusiones: la lactatemia perioperatoria constituye un indicador temprano de supervivencia en estos pacientes. Los valores de lactato sérico durante la circulación extracorpórea fueron mayores cuando se emplea ringer lactato en las soluciones de cebado. El tipo de cardiopatía no constituye un factor predictor de supervivencia.

Introduction: Lactic acid is considered as an early indicator of survival and/or mortality in patients with cardiovascular surgical procedures. Objectives: To determine behavior of lactic acid in arterial blood during pediatric cardiac surgery, and the prognosis of such patients when are discharged of intensive care unit. Material and Methods: We made a prospective, analytical and observational study of 55 pediatric patients undergo extracorporeal circulation in "William Soler" Children Cardiac Center. We took samples of arterial blood at the end of anesthesia induction (initial lactic acid), extracorporeal pre-circulation, and at the end of it. We considered the hyperlactacidemia when the lactic acid value in arterial blood was higher 3 mmol/L during the immediate intra- and postoperative period. Results: Lactic acid quantization in different times of surgical procedure was significant for the initial value of intraoperative period, and that of the extracorporeal circulation, but not for its relation with the value at the end of it. It was possible to demonstrate that the type of cardiopathy was not associated with the discharge status. Conclusions: Perioperative lactacidemia is an early indicator of survival in these patients. Serum lactate values during extracorporeal circulation were higher when it is used lactated ringer in primer solutions. The type of cardiopathy is not a predictor factor of survival.

La lactatemia cmmo pronostico inmediato de supevivencia en la cirugia cardiovaascular pediatrica a corazon abierto / Lactathemia as an immediate prognosis value of survival in open heart pediatric surgery

El ácido láctico ha sido considerado en los últimos años, como un indicador temprano de supervivencia y/o mortalidad en pacientes sometidos a cirugía cardíaca a corazón abierto para la corrección quirúrgica de sus cardiopatías congénitas. La apreciación de la oxigenación tisular durante la anemia aguda que se desarrolla durante la circulación extracorpórea de nuestros pacientes pediátricos con cardiopatías congénitas constituye la razón principal para evaluar si un paciente está o no adecuadamente perfundido, por lo que la evaluación seriada del ácido láctico en sangre arterial debe ser estimada en este tipo de cirugía. Teniendo en cuenta esto, decidimos realizar esta revisión sobre la lactatemia, en pacientes que van a ser sometidos a circulación extracorpórea,y garantizarles un mejor pronóstico. Se considera hiperlactatemia cuando su valor en sangre supera los 3 mmol/L.

The lactic acid has been considered in the last years, as an early indicator of survival and/or mortality in subjected patients to heart surgery to open heart for the surgical correction of its congenital cardiopathies. The appreciation of the tissular oxygenation during the anemia sharp normovolémica that is developed during the circulation extracorpórea of our pediatric patients with congenital cardiopathies constitutes the main reason to evaluate if a patient this or not appropriately perfused, for that the evaluation of the lactic acid in blood arterial debit side to be estimated in this surgery type. Keeping in mind this decides to carry out this revision on the lactatemia, in patients that will be subjected to circulation extracorpórea, guaranteeing them a better presage. It is considered hiperlactatemia when its value in blood overcomes the 3 mmol/L.

Relationship between the number of circulating endothelial cells and blood lipid level in critically ill patients and their clinical significance / 西安交通大学学报(医学版)

Objective To study the changes of the number of circulating endothelial cells (CEC) and blood lipid level in critically ill patients and their clinical significance. Methods The CECs were separated by using isopyknic centrifugation method and blood lipid level was determined in 35 critically ill patients and 20 healthy controls. CEC number and blood lipid level were compared between the two groups. And the correlation of CEC number and blood lipid level was analyzed. Clinical data from the critically ill patients was evaluated with acute physiology and chronic health evaluation Ⅱ; the correlation analysis of APACHEⅡscore, CEC number and blood lipid level was made. Results CEC number in the critically ill patients was significantly higher than that in healthy controls(P